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Automatic sequential fluid handling with multilayer microfluidic sample isolated pumping.

Jixiao Liu¹, Hai Fu¹, Tianhang Yang¹

¹Department of Fluid Control and Automation, Harbin Institute of Technology , Harbin 150001, China.

Biomicrofluidics

|October 22, 2015

Summary

This summary is machine-generated.

A new multilayer microfluidic sample isolated pumping (mμSIP) method enables bubble-free, controlled liquid handling for quantitative biology and bioassays. This mass-producible technology offers reliable fluidic actuation for microfluidic systems.

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Area of Science:

Biotechnology
Analytical Chemistry
Microfluidics

Background:

Sequential fluid handling is crucial for quantitative biology, analytical chemistry, and bioassays.
Current microfluidic systems face limitations in reliable, efficient, and mass-producible pumping methods.

Purpose of the Study:

To introduce a novel, bubble-free, and unified liquid handling method for microfluidic systems.
To develop a technology compatible with large-scale manufacturing for microfluidic applications.

Main Methods:

The multilayer microfluidic sample isolated pumping (mμSIP) method utilizes a selective permeable membrane.
This membrane separates fluidic and pneumatic layers, enabling controlled air diffusion for pressure variation.
Characterization involved analyzing fluidic actuation with varying parameters.

Main Results:

The mμSIP method demonstrated consistent, bubble-free liquid pumping.
Observed flow rate ranged from 0.013 to 0.097 μl/s.
A proof-of-concept automatic sequential fluid handling system for digital and immunoassays was successfully demonstrated.

Conclusions:

The mμSIP technology provides a unified pumping and control solution for microfluidic assays.
This method is suitable for functional microfluidic assays requiring minimal operations.
The mμSIP technology enriches the microfluidic toolbox and supports future innovations.