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RmtC and RmtF 16S rRNA Methyltransferase in NDM-1-Producing Pseudomonas aeruginosa

Mohibur Rahman, Kashi Nath Prasad, Ashutosh Pathak

    Emerging Infectious Diseases
    |October 22, 2015
    PubMed

    Insights

    We found 16S rRNA methyltransferases RmtC and RmtF in Pseudomonas aeruginosa isolates carrying the blaNDM-1 gene. These resistance genes were located on the chromosome, highlighting the need for strategies to limit the spread of these resistant bacteria.

    Area of Science:

    • Microbiology
    • Genetics
    • Antimicrobial Resistance

    Background:

    • Pseudomonas aeruginosa is an opportunistic pathogen.
    • The blaNDM-1 gene confers resistance to carbapenem antibiotics.
    • 16S rRNA methyltransferases are enzymes that confer high-level aminoglycoside resistance.

    Purpose of the Study:

    • To investigate the presence and location of 16S rRNA methyltransferases in blaNDM-1-positive Pseudomonas aeruginosa isolates.
    • To understand the genetic context of these resistance genes.

    Main Methods:

    • Screening of 38 blaNDM-1-positive Pseudomonas aeruginosa isolates for 16S rRNA methyltransferases.
    • Gene localization studies (chromosomal vs. plasmid).

    Main Results:

    • RmtC was detected in 3 isolates.
    • One isolate harbored both RmtC and RmtF.
    • The rmtC and rmtF genes were found to be chromosomally located alongside the blaNDM-1 gene.
    • The 38 isolates were determined to be clonally unrelated.

    Conclusions:

    • The co-location of blaNDM-1, rmtC, and rmtF on the chromosome of clonally distinct Pseudomonas aeruginosa isolates is a significant finding.
    • This suggests independent acquisition events or efficient chromosomal integration mechanisms.
    • Effective strategies are crucial to curb the dissemination of these multidrug-resistant isolates.
    Keywords:
    IndiaLucknowNDM-1Pseudomonas aeruginosaaminoglycosidesantimicrobial resistancebacteriaimipenemmeropenemmethyltransferases

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