[Coupled Cytotoxicity of C60 Nano-Crystal Particle with Cu2+ to the Mouse Peritoneal Macrophage RAW 264.7]

This work evaluated the cytotoxicity effect of nC60 nano-crystal particle associated with Cu2+ by using mouse peritoneal microphage RAW264. 7 as the test cell line. The results showed that when exposed to nC60 of 6. 6 mg . L-1 and 9. 9 mg . L-1 for 24h, the cell viability decreased 45% and 70% , respectively, and had obvious time-response and dose-response. It was found that Cu2, could reduce the toxicity of nC60 on RAW 264. 7. The cell viability reduced to 25% when exposed to 6. 6 mg . L-1 nC60 with 2 mg . L-1 Cu2+, and reduced to 15% when exposed to 9. 9 mg . L-1 nC60 with 5 mg . L-1 Cu2+. The nC60 could adsorb Cu2+ and the adsorption isotherm was fitted to the Langmuir adsorptiqn isotherm. The adsprption of Cu2+ on the surface of nC60 may decrease the cytotoxicity nC60 on RAW 264.7.