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Total triterpenoids from Ganoderma Lucidum suppresses prostate cancer cell growth by inducing growth arrest and

Tao Wang1,2, Zi-Ping Xie3, Zhan-Sen Huang4

  • 1Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
|October 23, 2015
PubMed

Insights

Ganoderma Lucidum triterpenoids (GLT) inhibit prostate cancer cell growth by inducing apoptosis and cell cycle arrest. These findings suggest Ganoderma Lucidum as a potential therapeutic for prostate cancer.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Prostate cancer remains a leading cause of cancer-related deaths globally.
  • Novel therapeutic strategies are crucial for effective prostate cancer treatment.
  • Ganoderma lucidum has traditional medicinal uses, but its specific anti-cancer mechanisms require elucidation.

Purpose of the Study:

  • To investigate the anti-cancer effects of Ganoderma lucidum triterpenoids (GLT) on human prostate cancer cell lines.
  • To elucidate the molecular mechanisms underlying GLT-induced inhibition of prostate cancer cell growth.
  • To assess the potential of GLT as a therapeutic agent for prostate cancer.

Main Methods:

  • Utilized human prostate cancer cell lines (LNCaP, 22Rv1, PC-3, DU-145) and a normal prostatic epithelial cell line (BPH).
  • Treated cells with varying doses and durations of GLT.
  • Analyzed cell viability, apoptosis, and cell cycle via flow cytometry and chemical assays.
  • Assessed gene expression and DNA binding using real-time PCR and Western blotting.

Main Results:

  • GLT demonstrated dose-dependent inhibition of prostate cancer cell growth.
  • GLT induced apoptosis through activation of Caspase-9 and Caspase-3.
  • GLT caused cell cycle arrest at the G1 phase, involving p21 up-regulation and CDK4/E2F1 down-regulation.

Conclusions:

  • GLT effectively suppresses prostate cancer cell proliferation by inducing apoptosis and cell cycle arrest.
  • The molecular pathways involve caspase activation and modulation of cell cycle regulatory proteins.
  • Ganoderma lucidum triterpenoids show promise as a potential therapeutic for prostate cancer.
Keywords:
Ganoderma Lucidum triterpenoidsapoptosiscell cycle arrestprostate cancer

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