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Updated: Mar 31, 2026

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
Published on: January 9, 2018
Cell-autonomous requirement for TCF1 and LEF1 in the development of Natural Killer T cells
Rosa Berga-Bolaños1, Wandi S Zhu1, Farrah C Steinke2
1Immune Cells and Inflammation Section, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, United States.
Abstract:
Natural killer T (NKT) cells develop from common CD4(+) CD8(+) thymocyte precursors. Transcriptional programs that regulate the development of NKT cells in the thymus development remain to be fully delineated. Here, we demonstrate a cell-intrinsic requirement for transcription factors TCF1 and LEF1 for the development of all subsets of NKT cells. Conditional deletion of TCF1 alone results in a substantial reduction in NKT cells. The remaining NKT cells are eliminated when TCF1 and LEF1 are both deleted. These data reveal an essential role for TCF1 and LEF1 in development of NKT cells.
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