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Updated: Mar 31, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
Published on: September 20, 2016
When the Label Matters: Adsorption of Labeled and Unlabeled Proteins on Charged Surfaces
Julia Romanowska1, Daria B Kokh1, Rebecca C Wade1,2,3
1Molecular and Cellular Modeling Group, Heidelberg Institute for Theoretical Studies , 69117 Heidelberg, Germany.
Abstract:
Fluorescent labels are often attached to proteins to monitor binding and adsorption processes. Docking simulations for native hen egg white lysozyme (HEWL) and HEWL labeled with fluorescein isothiocyanate show that these adsorb differently on charged surfaces. Attachment of even a small label can significantly change the interaction properties of a protein. Thus, the results of experiments with fluorescently labeled proteins should be interpreted by modeling the structures and computing the interaction properties of both labeled and unlabeled species.
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