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Updated: Sep 21, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
A programmable Y-shaped junction scaffold-mediated modular and cascade amplification strategy for the one-step,
Shufeng Liu1, Hongwei Gong, Xinya Sun
1Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao 266042, China. sliu@qust.edu.cn.
Abstract:
The programmable DNA polymerization across the two branches of the assembled Y-shaped junction was ingeniously manipulated for modular target recycling and cascade lambda exonuclease cleavage, which afforded the one-pot, isothermal and ultrasensitive detection of target DNA. A low detection limit of 28.2 fM of target DNA with an excellent selectivity could be obtained.
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