Related Experiment Video
Updated: Mar 31, 2026

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
Published on: April 18, 2014
Alternative splicing of two translesion synthesis DNA polymerases from Arabidopsis thaliana
María Jesús Santiago1, Encarna Alejandre-Durán1, Manuel Ruiz-Rubio1
1Departamento de Genética, Facultad de Ciencias, Edificio Gregor Mendel, Campus Rabanales, Universidad de Córdoba, Spain.
Abstract:
DNA damages can be removed by different repair processes, but lesions sometimes remain and block DNA replication. Specialized polymerases are needed to overcome this difficulty. In Arabidopsis, AtPOLH and AtREV1 genes code for two polymerases that are involved in replication of damaged DNA. Alternative splicing was detected in both genes. Complementation analysis of the alternative splicing forms in Saccharomycescerevisiae showed that the C-terminal extreme of AtPOLH protein is essential for recovering wild type UV viability in Rad30 deficient strain. None of the alternative AtREV1 forms recovered the yeast wild type phenotype of Rev1 deficient yeast strains after UV light irradiation or methyl methane sulphonate exposition, suggesting that AtREV1 may not be able to interact with other yeast specific proteins needed for DNA translesion synthesis.
Related Concept Videos
Translesion DNA Polymerases
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Alternative RNA Splicing
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
RNA Splicing
Homologous Recombination
DNA-only Transposons
The donor site from where the transposon is excised is either degraded or...

