Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of genetic damage and mutagenic properties of chlorpyrifos and glyphosate.

Annals of agricultural and environmental medicine : AAEM·2026
Same author

Systemic toxicity and immunotoxicity studies of glucose oxidase-loaded extracellular vesicles derived from lung cancer cells.

Frontiers in immunology·2026
Same author

SLC30A1, SLC30A5, and SLC30A9 transporters play crucial role in ligand-independent activation of ESR1 signalling in breast cancer cells via modulation of AKT activity by zinc.

Metallomics : integrated biometal science·2026
Same author

Citrate silver nanoparticles modulate estrogen signaling in estradiol-supplemented ER-positive breast cancer cells.

Molecular and cellular endocrinology·2026
Same author

Porous Curdlan-Whey Protein Isolate Scaffolds Obtained by Combined Method for Cartilage Tissue Engineering Application.

Materials (Basel, Switzerland)·2026
Same author

Comparative impact of polystyrene, rice bag-derived high-density polyethylene nanoparticles, and polystyrene-silver nanoparticle interactions in a 28-day in vivo study in male and female Wistar rats.

Scientific reports·2026

Related Experiment Video

Updated: Mar 31, 2026

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity
08:37

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

Published on: October 25, 2016

7.5K

Glucose availability determines silver nanoparticles toxicity in HepG2.

Mariusz Zuberek1, Dominika Wojciechowska2, Damian Krzyzanowski3

  • 1Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237, Lodz, Poland. zuberek.mariusz@gmail.com.

Journal of Nanobiotechnology
|October 24, 2015
PubMed
Summary

Glucose availability significantly impacts silver nanoparticle (AgNP) toxicity. Lower glucose levels enhance antioxidant defenses, reducing AgNP-induced oxidative stress and toxicity in cancer cells.

More Related Videos

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice
07:48

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice

Published on: October 13, 2018

8.1K
New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells
06:34

New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells

Published on: October 9, 2017

9.6K

Related Experiment Videos

Last Updated: Mar 31, 2026

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity
08:37

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

Published on: October 25, 2016

7.5K
A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice
07:48

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice

Published on: October 13, 2018

8.1K
New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells
06:34

New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells

Published on: October 9, 2017

9.6K

Area of Science:

  • Nanotoxicology
  • Cellular Respiration
  • Oxidative Stress

Background:

  • Nanomaterial toxicity is increasingly linked to oxidative stress.
  • Silver nanoparticles (AgNPs) are a common model for studying nanoparticle toxicity.
  • Cancer cells' high reliance on glucose suggests glucose availability may influence nanoparticle toxicity.

Purpose of the Study:

  • To investigate the role of cellular respiration in AgNP-induced oxidative stress and toxicity.
  • To determine how glucose availability affects AgNP toxicity in HepG2 cancer cells.

Main Methods:

  • HepG2 cells were cultured in high (25 mM) or low (5.5 mM) glucose media and treated with 20 nm AgNPs.
  • AgNP toxicity was assessed using neutral red assay.
  • Mitochondrial hydrogen peroxide (H2O2) generation, gene expression of oxidative stress markers, and antioxidant enzyme activity were measured.

Main Results:

  • AgNPs induced dose-dependent H2O2 generation and toxicity.
  • AgNP toxicity was significantly lower in low glucose conditions compared to high glucose.
  • Low glucose upregulated endogenous antioxidant defenses, mitigating AgNP-induced reactive oxygen species (ROS) generation and toxicity.

Conclusions:

  • Glucose availability modulates AgNP toxicity by influencing antioxidant defense mechanisms.
  • Enhanced oxidative phosphorylation and ROS generation in mitochondria are linked to AgNP toxicity.
  • NPs toxicity is strongly associated with intracellular respiration and mitochondrial function.