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The Rate of Inducible MLSB Resistance in the Methicillin-Resistant Staphylococci Isolated From Clinical Samples

Türkan Toka Özer1

  • 1Department of Medical Microbiology, Faculty of Medicine, Mevlana University, Konya, Turkey. tozer73@hotmail.com.

Journal of Clinical Laboratory Analysis
|October 24, 2015
PubMed
Abstract

Insights

Macrolide-lincosamide-streptogramin B (MLSB) resistance is a growing problem in staphylococcal infections. The D-test effectively identifies this resistance in clinical isolates, aiding treatment decisions.

Area of Science:

  • Clinical Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Staphylococci are common causes of nosocomial and community infections.
  • Methicillin-resistant staphylococci (MRS) resist beta-lactam antibiotics, necessitating alternative treatments like macrolides and lincosamides.
  • Resistance to these alternative antibiotics can cause treatment failure.

Purpose of the Study:

  • To determine the prevalence of macrolide-lincosamide-streptogramin B (MLSB) resistance in staphylococcal isolates.
  • To evaluate the utility of the D-test for detecting MLSB resistance.

Main Methods:

  • Seventy-one methicillin-resistant Staphylococcus isolates (MRS) were analyzed.
  • Conventional methods were used for staphylococci identification.
  • Kirby-Bauer disk diffusion method and D-test were performed according to CLSI criteria.

Main Results:

  • Overall, 28.1% of staphylococcal isolates were D-test positive for MLSB resistance.
  • 16.6% of methicillin-resistant Staphylococcus aureus (MRSA) and 29.2% of methicillin-resistant coagulase-negative staphylococci (MR-CNS) were D-test positive.
  • Inducible clindamycin resistance was more prevalent in MR-CNS.

Conclusions:

  • Routinely detecting MLSB resistance is crucial due to increasing resistant staphylococcal infections.
  • The D-test is a cost-effective and reliable method for identifying MLSB resistance in clinical microbiology laboratories.
Keywords:
D-testMLSBMLSB resistanceStaphylococcus

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