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Updated: Mar 31, 2026
10:18
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
Published on: May 28, 2019
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From gene editing to genome reconstitution: evolving techniques in yeast
1State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Yi Chuan = Hereditas
|October 27, 2015
Summary
Genome editing techniques, originating from homologous recombination repair of DNA double-strand breaks (DSBs), have evolved significantly. This review traces their history from early methods to modern genome synthesis for potential mammalian applications.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Homologous recombination is a primary DNA double-strand break (DSB) repair pathway in eukaryotes.
- Gene editing in eukaryotes was pioneered in S. cerevisiae over 30 years ago using homologous DNA fragments.
- The introduction of DSBs is fundamental to all current genome editing methodologies.
Purpose of the Study:
- To provide a historical overview of genome editing techniques.
- To trace the evolution from early methods to advanced genome synthesis and reconstitution.
- To offer insights into the developmental trends of genome editing.
Main Methods:
- Review of historical gene editing techniques.
- Analysis of methods based on inducing DNA double-strand breaks (DSBs).
- Examination of advancements towards genome synthesis and reconstitution.
Main Results:
- Established the historical progression of genome editing technologies.
- Highlighted the central role of DSB induction across different editing strategies.
- Demonstrated the evolution towards complex genome manipulation.
Conclusions:
- Genome editing techniques have a rich history rooted in DNA repair mechanisms.
- The field has advanced from basic sequence alteration to sophisticated genome synthesis.
- This review serves as a reference for future genome editing developments, particularly in mammals.
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