Related Experiment Video
Updated: Mar 31, 2026

A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl
Published on: November 28, 2014
SERPINB12 Is a Slow-Binding Inhibitor of Granzyme A and Hepsin
Jason Z Niehaus1, Mark T Miedel1, Misty Good1
1Department of Pediatrics and ‡Cell Biology and Physiology, University of Pittsburgh School of Medicine and The Children's Hospital of Pittsburgh of UPMC , 4401 Penn Avenue, Pittsburgh, Pennsylvania 15224, United States.
Abstract:
The clade B/intracellular serpins protect cells from peptidase-mediated injury by forming covalent complexes with their targets. SERPINB12 is expressed in most tissues, especially at cellular interfaces with the external environment. This wide tissue distribution pattern is similar to that of granzyme A (GZMA). Because SERPINB12 inhibits trypsin-like serine peptidases, we determined whether it might also neutralize GZMA. SERPINB12 formed a covalent complex with GZMA and inhibited the enzyme with typical serpin slow-binding kinetics. SERPINB12 also inhibited Hepsin. SERPINB12 may function as an endogenous inhibitor of these peptidases.
More Related Videos
Related Concept Videos
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Caspases
Inhibitors of Viral Protein Synthesis
The JAK-STAT Signaling Pathway
Inhibitors of Gram-positive Cell Wall Synthesis
Actin Filament Depolymerization
In F-actin, the ADF/cofilin proteins...

