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Updated: Mar 31, 2026
A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity
Published on: June 11, 2019
Comparison of innate immunity mediators in peritoneal fluid and spleen between young and aged rats
Young Joon Choi1, Seung Geun Yeo2, Young Il Kim3
1Department of Obstetrics and Gynecology, School of Medicine, Kyung Hee University, Seoul, Korea.
Background:
Aging of the innate immune system can result in a wide array of decreasing biological function.
Aim:
We investigated differences between young and old mice in the expression of the pattern-recognition receptors (PRRs).
Methods:
mRNA levels for PRRs were quantified and compared in peritoneal fluid and spleens from old (36 months old) and young (1 month old) Wistar white rats (n = 8/group) using real-time reverse transcription-polymerase chain reaction.
Results:
In old rats, TLR-5 and -7 were decreased in peritoneal fluid, whereas TLR 4/6/9 and Syk were increased in the spleen (p < 0.05). In young rats, TLR 2/4, dectin-1, and Trem-1 were increased in peritoneal fluid and decreased in the spleen (p < 0.05), but TLR 1/3/7/9/10 and Syk were vice versa (p < 0.05).
Discussion:
Several parameters related to innate immunity may change with aging.
Conclusion:
Different expressions of mRNA for several PRRs are suggesting changes in innate immune responses in association with aging.
