Related Experiment Video
Updated: Mar 31, 2026
Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
Published on: May 16, 2017
Brain morphologic changes in asymptomatic C9orf72 repeat expansion carriers
Renée Walhout1, Ruben Schmidt1, Henk-Jan Westeneng1
1From the Departments of Neurology (R.W., R.S., H.J.W., E.V., M.S., W.v.R., M.A.v.E., J.H.V., L.H.v.d.B.), Psychiatry (M.A.d.R., M.P.v.d.H.), and Radiology (J.H.), Brain Center Rudolf Magnus, University Medical Center Utrecht, the Netherlands.
Objective:
To investigate possible effects of the C9orf72 repeat expansion before disease onset, we assessed brain morphology in asymptomatic carriers.
Methods:
Aiming to diminish the effects of genetic variation between subjects, apart from the C9orf72 repeat expansion, 16 carriers of the repeat expansion were compared with 23 noncarriers from the same large family with a history of amyotrophic lateral sclerosis (ALS). Cortical thickness, subcortical volumes, and white matter connectivity, as assessed from high-resolution T1-weighted and diffusion-weighted MRIs, were evaluated. For comparison, we included 14 C9orf72 carriers with ALS and 28 healthy, unrelated controls.
Results:
We found temporal, parietal, and occipital regions to be thinner (p < 0.05) and the left caudate and putamen to be smaller (p < 0.05) in asymptomatic carriers compared with noncarriers. Cortical thinning of the primary motor cortex and decreased connectivity of white matter pathways (global, corticospinal tract, and corpus callosum) were observed in patients with C9orf72-associated ALS, but not in asymptomatic carriers.
Conclusions:
Asymptomatic C9orf72 carriers show cortical and subcortical differences compared with noncarriers from the same family, possibly effects of the C9orf72 repeat expansion on the brain. Of note, changes in the primary motor regions and motor-related tracts were found exclusively in patients with ALS, indicating that such motor changes may be a disease phenomenon.
