Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Histidine to glutamine substitutants, a regulated process that impacts cell survival and suggests histidine shortage in cancer.

Cell death discovery·2026
Same author

In vitro Evaluation of Immunomodulatory and Antioxidant Potential of Isolated Lactic Acid Bacteria from Iranian Dairy Products: An Insight into beneficial potential of cheese-derived isolates.

Probiotics and antimicrobial proteins·2026
Same author

Comparative transcriptomics reveals shared stress and repair-associated pathway modules in proximal tubule cells exposed to platinum chemotherapeutics.

Toxicology·2026
Same author

Diagnostic Accuracy of Interleukin 27 for the Diagnosis of Pleural Tuberculosis.

Tanaffos·2026
Same author

Update on the Efficacy of Vaccination on Host Immunity to Influenza Virus Infection.

International archives of allergy and immunology·2026
Same author

MiR-146a-armed exosomes reverse immunosuppressive networks in NSCLC by dual-targeting of MYD88/STAT3 and PD-L1 axes.

International immunopharmacology·2026

Related Experiment Video

Updated: Mar 31, 2026

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System
06:07

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System

Published on: October 22, 2020

7.9K

Cigarette smoke differentially modulates dendritic cell maturation and function in time.

Masoumeh Ezzati Givi1,2, Gert Folkerts1, Gerry T M Wagenaar3

  • 1Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, PO BOX 80082, 3508, TB, Utrecht, The Netherlands.

Respiratory Research
|October 27, 2015
PubMed
Summary
This summary is machine-generated.

Cigarette smoke extract (CSE) initially promotes dendritic cell (DC) maturation but prolonged exposure suppresses DC function and immune responses. This study reveals CSE

More Related Videos

Author Spotlight: Development and Characterization of an In Vitro Model to Study Chronic Cigarette Smoke Exposure and Its Impact on Airway Epithelial Cells in COPD Research
09:07

Author Spotlight: Development and Characterization of an In Vitro Model to Study Chronic Cigarette Smoke Exposure and Its Impact on Airway Epithelial Cells in COPD Research

Published on: July 12, 2024

2.0K
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
06:09

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Published on: June 22, 2016

24.2K

Related Experiment Videos

Last Updated: Mar 31, 2026

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System
06:07

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System

Published on: October 22, 2020

7.9K
Author Spotlight: Development and Characterization of an In Vitro Model to Study Chronic Cigarette Smoke Exposure and Its Impact on Airway Epithelial Cells in COPD Research
09:07

Author Spotlight: Development and Characterization of an In Vitro Model to Study Chronic Cigarette Smoke Exposure and Its Impact on Airway Epithelial Cells in COPD Research

Published on: July 12, 2024

2.0K
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
06:09

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Published on: June 22, 2016

24.2K

Area of Science:

  • Immunology
  • Cell Biology
  • Toxicology

Background:

  • Dendritic cells (DCs), professional antigen-presenting cells (APCs), are crucial for regulating immune responses.
  • DCs transition from immature antigen-capturing cells to mature antigen-presenting cells.
  • The impact of cigarette smoke on DC-mediated inflammation is not well understood.

Purpose of the Study:

  • To investigate the modulatory effects of cigarette smoke extract (CSE) on DC differentiation, maturation, and function.
  • To elucidate the mechanisms by which CSE influences DC-mediated immune responses.

Main Methods:

  • Primary murine DCs were cultured from bone marrow cells and analyzed for maturation markers (CD11c, MHCII, CD86, CD40, CD83) via flow cytometry.
  • Murine DCs and human L428 cells were co-cultured with CSE.
  • Functional assays included FITC-dextran uptake, cytokine production (IL-12, IL-6, TNF-α), and T cell activation assessment.

Main Results:

  • Short-term CSE exposure (~24h) induced DC maturation, upregulating surface markers and pro-inflammatory cytokines while reducing antigen uptake.
  • Long-term CSE exposure suppressed DC development and function in a time- and concentration-dependent manner, reducing key surface markers and T cell activation.
  • CSE impaired DC endocytosis without affecting cell viability and suppressed CD54 expression in human DCs.

Conclusions:

  • CSE differentially modulates DC function and maturation, with short-term exposure promoting maturation and long-term exposure causing suppression.
  • The suppressive effects of CSE on DC function may impair immune responses to infections.
  • Understanding CSE's impact on DCs is critical for addressing cigarette smoke-related immune dysregulation.