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Identification of fyn-encoded proteins in normal human blood cells
Y Kawakami1, M Furue, T Kawakami
1Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
Abstract:
We have previously reported that carboxyl terminal truncations of the normal human fyn gene, a member of the src subfamily, can transform immortal mouse fibroblasts to full malignancy. In search of evidence which suggests the possible activation of the human fyn gene, we have screened DNAs and RNAs from a number of human tumor cell lines. The results indicate that the fyn gene is not frequently changed grossly in the naturally occurring tumors. To establish a basis for understanding the physiological function(s) of this highly conserved gene, we have prepared specific antibodies to the fyn-encoded proteins. Using them we have shown the presence of fyn proteins in human lymphocytes and monocytes and their protein-tyrosine kinase activity.
Insights
The human fyn gene, a src subfamily member, does not appear grossly altered in most human tumors. However, fyn proteins are present in lymphocytes and monocytes, exhibiting protein-tyrosine kinase activity.
Area of Science:
- Molecular Biology
- Oncology
- Immunology
Background:
- Carboxyl terminal truncations of the human fyn gene can induce malignancy in mouse fibroblasts.
- The src subfamily includes the fyn gene, a highly conserved gene with potential roles in cell signaling.
Purpose of the Study:
- To investigate potential alterations and activation of the human fyn gene in human tumor cell lines.
- To understand the physiological functions of the fyn gene by characterizing its protein products.
Main Methods:
- Screening of DNA and RNA from human tumor cell lines for fyn gene changes.
- Preparation of specific antibodies against fyn-encoded proteins.
- Detection and activity assay of fyn proteins in human immune cells.
Main Results:
- The fyn gene does not show frequent gross changes in naturally occurring human tumors.
- Fyn proteins were detected in human lymphocytes and monocytes.
- These fyn proteins possess protein-tyrosine kinase activity.
Conclusions:
- The human fyn gene is not commonly altered at the gross level in human tumors.
- Fyn proteins are present in immune cells and possess enzymatic activity, suggesting a role in immune cell function.