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Generating Transgenic Mouse Models for Studying Celiac Disease.

Josephine M Ju¹, Eric V Marietta^1,2,3, Joseph A Murray^4,5,6

¹Department of Immunology, Mayo Clinic Rochester, Rochester, MN, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 27, 2015

Summary

This summary is machine-generated.

Current animal models for celiac disease, including Human Leukocyte Antigen (HLA) transgenic mice and interleukin-15 (IL-15) overexpressing mice, help study disease mechanisms. These models investigate specific factors contributing to gluten-dependent enteropathy.

Keywords:

Animal model Celiac Gliadin Gluten In vivo MHC II Mouse

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Area of Science:

Immunology
Gastroenterology
Animal Models

Background:

Celiac disease pathogenesis involves complex interactions.
Human Leukocyte Antigen (HLA) class II molecules are strongly associated with celiac disease.
Interleukin-15 (IL-15) plays a critical role in the inflammatory cascade.

Purpose of the Study:

To provide an overview of current animal models for studying celiac disease.
To highlight the development and application of transgenic mouse models.
To explore the role of specific factors in gluten-sensitive enteropathy.

Main Methods:

Generation of Human Leukocyte Antigen (HLA) transgenic mouse models by replacing endogenous MHC class II genes with disease-susceptible HLA class II alleles (DQ2 or DQ8).
Development of transgenic mice overexpressing interleukin-15 (IL-15) to model chronic inflammation.
Utilizing nude mouse and rat models in germ-free facilities to study bacterial contributions to gluten-sensitive enteropathy.

Main Results:

Various reductionist animal models have been created to investigate specific factors in celiac disease.
Transgenic models allow for the functional interrogation of components involved in disease pathogenesis.
No single model currently encompasses all factors contributing to celiac disease.

Conclusions:

A diverse array of animal models exists for celiac disease research.
These models facilitate the study of specific pathogenic mechanisms.
Further research using these tools can advance understanding of celiac disease pathogenesis.