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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Spermatogenesis01:41

Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Related Experiment Video

Updated: Mar 31, 2026

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
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Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

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Advances in Isolation Methods for Spermatogonial Stem Cells.

Rui Zhang, Jin Sun, Kang Zou

    Stem Cell Reviews and Reports
    |October 27, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Spermatogonial stem cell (SSC) isolation techniques have seen limited advancement. This review covers SSC properties, historical sorting methods, recent progress, and isolation pros and cons to guide future reproductive biology research.

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    Serial Enrichment of Spermatogonial Stem and Progenitor Cells SSCs in Culture for Derivation of Long-term Adult Mouse SSC Lines
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    Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
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    Serial Enrichment of Spermatogonial Stem and Progenitor Cells SSCs in Culture for Derivation of Long-term Adult Mouse SSC Lines
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    Serial Enrichment of Spermatogonial Stem and Progenitor Cells SSCs in Culture for Derivation of Long-term Adult Mouse SSC Lines

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    Area of Science:

    • Reproductive Biology
    • Stem Cell Science

    Background:

    • Spermatogonial stem cells (SSCs) are crucial for male fertility.
    • Progress in SSC research is hindered by slow development of isolation techniques.

    Purpose of the Study:

    • To review the biological properties of SSCs.
    • To analyze the evolution of SSC sorting and identification techniques over the past decades.
    • To discuss the advantages and disadvantages of current SSC isolation methods.

    Main Methods:

    • Review of historical and recent literature on SSC sorting and identification.
    • Analysis of biological properties of SSCs.
    • Comparative discussion of SSC isolation techniques.

    Main Results:

    • Major SSC sorting and identification advancements occurred a decade ago and have seen minimal subsequent improvement.
    • Existing techniques are widely used but lack significant innovation.
    • Recent achievements in SSC sorting are summarized.

    Conclusions:

    • Further development of SSC isolation techniques is needed to accelerate progress in reproductive biology.
    • Understanding the limitations of current methods is key to future advancements.
    • This review provides insights to promote further research in spermatogonial stem cells.