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Updated: Mar 31, 2026

Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction
Published on: April 27, 2011
Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila
Qi He1, Christopher Roblodowski2
1Department of Biology, Brooklyn College, City University of New York, 209 Ingersoll Hall Extension, 2900 Bedford Avenue, Brooklyn, NY, 11210, USA. QHe@brooklyn.cuny.edu.
Abstract:
Using Drosophila actin-binding protein Dunc-115 as model system, this chapter describes a MARCM (mosaic analysis with a repressible cell marker)-based method for analyzing cytoskeletal components for their functions in the nervous system. Following a concise description about the principle, a step-by-step protocol is provided for generating the needed stocks and for histological analysis. Additional details and explanations have been given in the accompanying notes. Together, this should form a practical and sufficient recipe for performing at the single-cell-level loss-of-function and gain-of-function analyses of proteins associated with the cytoskeleton.
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