Related Experiment Video
Updated: Mar 31, 2026

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
Published on: October 14, 2022
HiFive: a tool suite for easy and efficient HiC and 5C data analysis
Michael Eg Sauria1, Jennifer E Phillips-Cremins2, Victor G Corces3
1Departments of Biology and Computer Science, Johns Hopkins University, Baltimore, MD, 21218, USA.
Abstract:
The chromatin interaction assays 5C and HiC have advanced our understanding of genomic spatial organization, but analysis approaches for these data are limited by usability and flexibility. The HiFive tool suite provides efficient data handling and a variety of normalization approaches for easy, fast analysis and method comparison. Integration of MPI-based parallelization allows scalability and rapid processing time. In addition to single-command analysis of an entire experiment from mapped reads to interaction values, HiFive has been integrated into the open-source, web-based platform Galaxy to connect users with computational resources and a graphical interface. HiFive is open-source software available from http://taylorlab.org/software/hifive/ .
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