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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
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Scanning Electrospray Microscopy with Nanopipets.

Elizabeth M Yuill1, Wenqing Shi1, John Poehlman1

  • 1Department of Chemistry, Indiana University , 800 E. Kirkwood Avenue, Bloomington, Indiana 47405, United States.

Analytical Chemistry
|October 27, 2015
PubMed
Summary
This summary is machine-generated.

Scanning electrospray microscopy (SESM) uses nanopipet electrospray for noncontact surface imaging. This method maps surface topography by analyzing electrospray current changes with distance.

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Area of Science:

  • Surface science
  • Microscopy techniques
  • Nanotechnology

Background:

  • Traditional microscopy methods can be contact-based or require vacuum environments.
  • Investigating surface topography in ambient conditions presents unique challenges.
  • Electrospray phenomena offer potential for novel imaging modalities.

Purpose of the Study:

  • To introduce and demonstrate Scanning Electrospray Microscopy (SESM) as a novel surface imaging technique.
  • To explore the use of electrospray from nanopipets for ambient, noncontact surface analysis.
  • To characterize the capabilities and limitations of SESM for surface topography investigation.

Main Methods:

  • Utilizing electrospray generated from nanopipets.
  • Employing a noncontact approach for sample interaction.
  • Measuring the distance dependence of electrospray current for feedback.
  • Performing approach curves, line scans, and image acquisition.
  • Characterizing salt deposition resulting from the SESM process.

Main Results:

  • SESM successfully generates images of surface topography.
  • The electrospray current shows a distance-dependent behavior useful for imaging.
  • Approach curves and line scans provide detailed topographical information.
  • Salt deposition from the electrospray process was observed and characterized.

Conclusions:

  • SESM is a viable ambient, noncontact method for surface topography investigation.
  • The distance dependence of electrospray current is a key parameter for SESM imaging.
  • Further studies can optimize SESM for various surface analysis applications.