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Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
Published on: June 10, 2018
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Cell Pluripotency Levels Associated with Imprinted Genes in Human
Liyun Yuan1, Xiaoyan Tang1, Binyan Zhang2
1Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, SIBS, CAS, Shanghai 200031, China.
Computational and Mathematical Methods in Medicine
|October 28, 2015
Summary
Induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) show differences in pluripotency. This study identifies a novel imprinted region linking microRNAs and imprinted genes, potentially explaining these differences.
Area of Science:
- Stem cell biology
- Epigenetics
- Genomics
Background:
- Pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), share similarities but exhibit controversial differences in pluripotency and differentiation capacity.
- MicroRNAs (miRNAs) are key regulators of gene expression and cellular processes, potentially holding insights into stem cell pluripotency.
Purpose of the Study:
- To investigate the relationship between miRNAs and imprinted genes in the context of cell pluripotency.
- To compare miRNA expression patterns in iPSCs and ESCs and link them with human imprinted genes.
Main Methods:
- Comprehensive analysis of total miRNA expression patterns in iPSCs and ESCs.
- Linking miRNA expression with human imprinted genes.
- Molecular network analysis of interacting genes.
Main Results:
- A novel CPA4-KLF14 imprinted region was identified within chromosomal homologous segments (CHSs), containing both imprinted genes and significantly expressed miRNAs.
- miRNA expression patterns in iPSCs and ESCs were analyzed and correlated with human imprinted genes.
- Network analysis revealed interactions between imprinted genes and genes enriched in cancer, cell death, survival, and tumor morphology pathways.
Conclusions:
- The identified CPA4-KLF14 imprinted region offers a new perspective on the functional differences in pluripotency between hiPSCs and hESCs.
- The interplay between miRNAs and imprinted genes in this region may contribute to understanding stem cell pluripotency and differentiation.
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