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Updated: Mar 31, 2026

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
Published on: August 5, 2020
Temporal and spatial control of gene expression in horticultural crops
Manjul Dutt1, Sadanand A Dhekney2, Leonardo Soriano3
1Citrus Research and Education Center, University of Florida , 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
Biotechnology offers plant breeders powerful tools for enhancing horticultural crops. This review explores plant promoters for precise gene expression control and crop improvement.
Area of Science:
- Plant biotechnology
- Horticultural science
- Molecular biology
Background:
- Biotechnology offers advanced tools for improving horticultural crop traits and addressing genetic challenges.
- The increasing availability of horticultural genome sequences facilitates DNA-based research and cultivar development.
- Promoters are crucial for regulating plant gene expression, with significant progress in their isolation and application.
Purpose of the Study:
- To review the temporal and spatial control of gene expression in horticultural crops.
- To examine the utilization of plant-derived promoters in horticultural crop improvement.
- To highlight the shift from discovery to implementation in horticultural plant biotechnology.
Main Methods:
- Review of current literature on plant promoters and their application in horticulture.
- Analysis of gene expression regulation in horticultural species.
- Evaluation of genetic transformation techniques in horticultural crops.
Main Results:
- Plant biotechnology provides effective tools for trait enhancement and genetic problem-solving in horticultural crops.
- The identification and application of plant promoters enable precise control over gene expression.
- Horticultural plant biotechnology has advanced from a research phase to practical implementation.
Conclusions:
- The use of native promoters from horticultural plants allows for precision breeding and crop enhancement.
- Advances in understanding and applying promoters are revolutionizing horticultural crop improvement.
- Horticultural plant biotechnology is increasingly utilizing genetic resources for targeted trait development.
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