Related Experiment Video
Updated: Mar 31, 2026
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
Published on: November 5, 2020
Development and characterization of 25 microsatellite primers for Ilex chinensis (Aquifoliaceae)
Wen-Wen Chen1, Zhen-Zhu Xiao1, Xin Tong1
1School of Ecological and Environmental Sciences, Tiantong National Station of Forest Ecosystem, Shanghai Key Laboratory for Urban Ecology and Restoration, East China Normal University, Shanghai 200241, People's Republic of China.
Premise Of The Study:
To evaluate genetic variation and structure of Ilex chinensis (Aquifoliaceae), a dioecious evergreen tree, we developed 25 microsatellite markers from its nuclear genome.
Methods And Results:
Based on the biotin-streptavidin capture method, 10 polymorphic and 15 monomorphic microsatellite markers were developed. Ten polymorphic loci were characterized by 87 individuals sampled from three populations located in Zhejiang Province and Shanghai, China. The number of alleles per locus varied from two to 12. The observed and expected heterozygosities were 0.0435-0.9032 and 0.3121-0.8343, respectively.
Conclusions:
These microsatellite markers can be useful for further genetic studies of I. chinensis populations, and so contribute to forest restoration and management.
More Related Videos
12:01A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
10:27A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Published on: April 20, 2018