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Intestinal lactic acid bacteria from Muscovy duck as potential probiotics that alter adhesion factor gene expression
1University Key Lab for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, China.
Genetics and Molecular Research : GMR
|October 28, 2015
Summary
Lactic acid bacteria from Muscovy ducks show probiotic potential, exhibiting resistance to bile and acid. These gut bacteria can help select strains for preventing diarrhea.
Area of Science:
- Microbiology
- Animal Science
- Gastroenterology
Background:
- The gut microbiome plays a crucial role in host health.
- Probiotics offer a promising avenue for preventing gastrointestinal disorders.
- Identifying novel probiotic sources is essential for developing effective interventions.
Purpose of the Study:
- To evaluate lactic acid bacteria (LABs) from Muscovy ducks as potential probiotics.
- To assess in vitro probiotic characteristics of duck-derived LAB isolates.
- To investigate the impact of LABs on host gene expression related to Caco-2 cell adhesion.
Main Methods:
- Isolation and identification of LABs using polymerase chain reaction.
- In vitro assessment of autoaggregation, surface charge, hydrophobicity, and tolerance to pH, bile, and protease.
- Analysis of F-actin, cell adhesion molecule (CAM), and E-cadherin (E-cad) mRNA expression in Caco-2 cells.
Main Results:
- LAB isolates demonstrated strong resistance to bile salts and acidic pH.
- Bacilli-shaped LAB isolates showed greater bacteriostatic effects compared to cocci.
- Treatment with a specific LAB isolate (CM3) and Salmonella enterica influenced F-actin, CAM, and E-cad mRNA levels.
Conclusions:
- Muscovy duck gut microflora is a valuable source of probiotic bacteria.
- Selected LAB strains possess characteristics suitable for probiotic applications.
- These findings contribute to establishing criteria for selecting probiotic strains for diarrhea prevention.
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