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Updated: Mar 31, 2026
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
Published on: February 10, 2022
The systematic analysis of protein-lipid interactions comes of age
Antoine-Emmanuel Saliba1, Ivana Vonkova2, Anne-Claude Gavin3
1Institute for Molecular Infection Biology and Core Unit Systems Medicine, University of Würzburg, Josef-Schneider-Strasse 2, D-97080 Würzburg, Germany.
Abstract:
Lipids tailor membrane identities and function as molecular hubs in all cellular processes. However, the ways in which lipids modulate protein function and structure are poorly understood and still require systematic investigation. In this Innovation article, we summarize pioneering technologies, including lipid-overlay assays, lipid pull-down assays, affinity-purification lipidomics and the liposome microarray-based assay (LiMA), that will enable protein-lipid interactions to be deciphered on a systems level. We discuss how these technologies can be applied to the charting of system-wide networks and to the development of new pharmaceutical strategies.
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