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Mallory-Denk bodies: Correlation with steatosis, severity, zonal distribution, and identification with ubiquitin
Serra Kayaçetin1, Metin Başaranoğlu
1Department of Pathology, Ankara Numune Training and Research Hospital, Ankara, Turkey. skayaetin@gmail.com.
Background/Aims:
Intermediate filament proteins contain few aggregates as their main component. Among those, Mallory-Denk bodies (MDBs) are by far the best recognized component. To identify the presence of MDBs in individuals having chronic liver disease and to evaluate the correlation among MDBs and steatosis as well as the severity and zonal distribution of hepatocyte balloon degeneration. Tertiary reference hospital.
Materials And Methods:
Three hundred consecutive liver specimens derived from our patients with chronic liver disease were included in the current study. Immunohistochemistry analysis was conducted on frozen liver biopsies fixed at room temperature with acetone anti-rabbit antibody to ubiquitin. In addition, histological activity was evaluated by the routine staining of liver biopsy sections with hematoxylin-eosin and periodic staining by acid-Schiff stain, reticulin, Masson trichrome, and iron. The presence of MDBs, steatosis, severity, and the zonal distribution of hepatocyte balloon degeneration were evaluated in every patient.
Results:
Histopathologic diagnosis were chronic hepatitis B (n=219), alcoholic steatohepatitis (n=23), non-alcoholic steatohepatitis (n=20), chronic hepatitis C (n=20), overlap syndrome (n=10), and primary biliary cirrhosis (n=8). The distribution of MDBs stained positive for ubiquitin was 80% in the overlap syndrome, 86% in chronic hepatitis B, and 100% in alcoholic steatohepatitis, NASH, chronic hepatitis C, and primary biliary cirrhosis. There was a correlation between the severity of steatosis and ubiquitin positivity, particularly in zone 2. A conspicuous correlation existed between the severity of hepatocyte balloon degeneration and ubiquitin positivity.
Conclusion:
These findings have demonstrated that the observation of MDB together with ubiquitin positivity will be helpful in the evaluation of the models of diagnosis, staging, and therapy in patients with chronic liver disease.
Insights
Mallory-Denk bodies (MDBs) are common in chronic liver disease and correlate with steatosis and balloon degeneration. Ubiquitin positivity aids in diagnosing and staging liver disease.
Area of Science:
- Hepatology
- Pathology
- Biochemistry
Background:
- Mallory-Denk bodies (MDBs) are protein aggregates found in hepatocytes.
- Their presence and significance in various chronic liver diseases require further investigation.
Purpose of the Study:
- To identify Mallory-Denk bodies (MDBs) in patients with chronic liver disease.
- To evaluate the correlation between MDBs, steatosis, and hepatocyte balloon degeneration severity and distribution.
Main Methods:
- Analysis of 300 liver biopsy specimens from patients with chronic liver disease.
- Immunohistochemistry for ubiquitin to detect MDBs.
- Histological evaluation including H&E, PAS, reticulin, Masson trichrome, and iron staining.
Main Results:
- MDBs (ubiquitin-positive) were found in 80-100% of chronic liver disease cases.
- A correlation was observed between steatosis severity and ubiquitin positivity, especially in zone 2.
- Significant correlation between hepatocyte balloon degeneration severity and ubiquitin positivity.
Conclusions:
- Ubiquitin positivity for MDBs is a valuable marker in chronic liver disease.
- These findings can aid in the diagnosis, staging, and therapeutic evaluation of chronic liver conditions.
