Sequences analyses and expression profiles in tissues and embryos of Japanese flounder (Paralichthys olivaceus) PRDM1
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Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
Published on: February 9, 2017
Conghui Liu1, Wei Liu1, Lin Fan1
1Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao, 266003, China.
Insights
Researchers identified the Po-prdm1 gene in Paralichthys olivaceus, revealing its widespread expression during embryonic development and in adult tissues. This suggests a conserved, pleiotropic regulatory role for PRDM1 (PRDI-BF1-RIZ1 homologous domain containing 1) in fish and mammals.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- PRDM1 (PRDI-BF1-RIZ1 homologous domain containing 1) is a known regulatory factor with diverse functions.
- Its specific role and expression patterns in teleosts are not fully elucidated.
Purpose of the Study:
- To identify and characterize the PRDM1 homolog (Po-prdm1) in Paralichthys olivaceus.
- To investigate the spatiotemporal expression patterns of Po-prdm1 during embryonic development and in adult tissues.
- To analyze the transcriptional regulation and conserved binding regions of Po-prdm1.
Main Methods:
- Gene identification and 5' regulatory region analysis.
- Real-time quantitative polymerase chain reaction (RT-qPCR) for expression analysis.
- In situ hybridization (ISH) for spatial expression patterns in embryos and tissues.
- Bioinformatic analysis of transcription factor binding sites.
Main Results:
- The Po-prdm1 gene and its 5' regulatory region were successfully identified in Paralichthys olivaceus.
- Po-prdm1 exhibited high expression during early embryonic development (gastrula to tail bud stages), peaking at mid-gastrula.
- Ubiquitous expression was detected in all adult tissues, with specific localization in oocytes and spermatogonia, indicating pleiotropic functions.
Conclusions:
- Po-prdm1 plays a significant role in the early development of Paralichthys olivaceus.
- The gene's ubiquitous expression and specific gonadal localization suggest conserved pleiotropic functions.
- Analysis of binding sites indicates a conserved regulatory mechanism for PRDM1 across teleosts and mammals.
Abstract:
PRDM1 (PRDI-BF1-RIZ1 homologous domain containing 1) appears to be a pleiotropic regulatory factor in various processes. It contains a PR (PRDI-BF1-RIZ1 homologous) domain protein and five zinc fingers. In the present study, a gene coding the homolog of prdm1 and the 5' regulatory region of prdm1 was identified from the Paralichthys olivaceus (denoted Po-prdm1). Results of real-time quantitative polymerase chain reaction amplification (RT-qPCR) and in situ hybridization (ISH) in embryos revealed that Po-prdm1 was highly expressed between the early gastrula and tail bud stages, with its expression peaking in the mid-gastrula stage, whereas the results of RT-qPCR and ISH in tissues demonstrated that Po-prdm1 transcripts were ubiquitously detected in all tissues, which indicates its pleiotropic function in multiple processes. ISH of gonadal tissues revealed that the transcripts were located in the nucleus and cytoplasm of the oocytes in the ovaries but only in the spermatogonia and not in the spermatocytes in the testes. The Po-prdm1 transcription factor binding sites and their conserved binding region among vertebrates were analyzed in this study. The combined results suggest that Po-PRDM1 has a conserved function in teleosts and mammals.
