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Characterization of the human cell line TE671
M R Stratton1, J Darling, G J Pilkington
1Section of Chemical Carcinogenesis, Chester Beatty Laboratories, Institute of Cancer Research, UK.
Carcinogenesis
|May 1, 1989
Summary
The TE671 cell line, previously thought to be medulloblastoma, is actually rhabdomyosarcoma. This is due to an N-ras gene mutation and shared characteristics with the RD cell line.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- The TE671 cell line is commonly used as a model for human medulloblastoma.
- Phenotypic characteristics of TE671 suggest a potential myoid origin.
Purpose of the Study:
- To investigate the true identity of the TE671 cell line.
- To determine the genetic basis for the TE671 cell line's characteristics.
Main Methods:
- Transfection of TE671 DNA into NIH 3T3 cells.
- Oligonucleotide probe analysis to detect N-ras gene mutations.
- Cytogenetic analysis and DNA fingerprinting.
Main Results:
- An activated N-ras gene with a point mutation at codon 61 was identified in TE671 cells.
- This mutation was also found in the human rhabdomyosarcoma cell line RD.
- Cytogenetic and DNA fingerprinting analyses showed TE671 and RD are derived from the same cell line.
Conclusions:
- The TE671 cell line is not a medulloblastoma model but shares identity with the RD rhabdomyosarcoma cell line.
- The observed properties of TE671 should be attributed to rhabdomyosarcoma, not medulloblastoma.