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Maize MeJA-responsive proteins identified by high-resolution 2-DE PAGE
Yuliang Zhang1, Kayla K Pennerman2, Fengshan Yang3
1Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
Data in Brief
|October 29, 2015
Summary
Methyl jasmonate (MeJA) treatment boosts maize plant defenses against pests. Proteomic analysis identified 62 MeJA-responsive proteins, aiding in understanding maize
Area of Science:
- Plant Biology
- Proteomics
- Biochemistry
Background:
- Methyl jasmonate (MeJA) is a plant hormone known to trigger defense responses.
- These plant defense mechanisms are crucial for protecting crops against insect and microbial threats.
Purpose of the Study:
- To investigate the changes in the maize leaf proteome following exposure to MeJA.
- To identify specific proteins involved in the plant's defense response.
Main Methods:
- High-resolution 2-DE gel electrophoresis was employed to separate and visualize proteins.
- Tandem mass spectrometry was used to sequence and identify MeJA-responsive proteins.
Main Results:
- Sixty-two proteins exhibiting altered expression levels in response to MeJA were identified.
- The proteomic data and protein identities were deposited in the PRIDE database (PXD001793).
Conclusions:
- The study successfully identified key proteins modulated by MeJA in maize.
- These findings contribute to understanding the molecular basis of maize defense against pests like the Asian corn borer.
