[Infrared fluorescent markers for microarray DNA analysis on biological microchip].

Area of Science:

• Molecular genetics
• Biochemistry
• Analytical chemistry

Context:

• Biological microchips offer advanced platforms for molecular genetic research.
• Detecting genetic mutations, such as in BRCA1/BRCA2 and CHECK2 genes, is crucial for breast cancer diagnostics.
• Traditional fluorescent detection methods can be limited by background noise and signal interference.

Purpose:

• To synthesize novel indotricarbocyanine dyes emitting in the near-infrared (IR) spectral region.
• To develop and validate a biochip-based system utilizing these IR dyes for genetic mutation detection.
• To enhance the informational capabilities and reliability of molecular genetic analyses.

Summary:

• New indotricarbocyanine dyes fluorescing in the near-infrared (IR) region were synthesized.
• A biochip-based system was developed to detect mutations in breast cancer-associated genes (BRCA1/BRCA2, CHECK2) using these IR dyes.
• The fluorescent label was incorporated during polymerase chain reaction (PCR) via IR dye-labeled primers.
• A dedicated IR spectral region analyzer was designed for image recording and processing.

Impact:

• The developed IR dyes enable genetic analysis in the IR spectral region, reducing background fluorescence.
• This leads to improved reliability and accuracy in medical diagnostic tests for breast cancer-related gene mutations.
• The synthesized dyes expand the capabilities of molecular genetic research on biological microchips.