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Classification of mouse submaxillary gland esteroproteases by their substrate specificities
1Department of Pathogenic, Biochemistry, Toyama Medical and Pharmaceutical University, Japan.
Electrophoresis
|March 1, 1989
Summary
Researchers identified eight distinct esteroprotease groups in male mouse submaxillary glands. These enzymes were classified by substrate specificity, inhibitor sensitivity, and androgen dependence, revealing their diverse functions.
Area of Science:
- Biochemistry
- Enzymology
- Proteomics
Background:
- Submaxillary glands produce various esteroproteases.
- Understanding these enzymes is crucial for salivary gland research.
- Previous classifications were limited.
Purpose of the Study:
- To comprehensively classify esteroproteases in male mouse submaxillary glands.
- To determine substrate specificities and regulatory mechanisms of these enzymes.
Main Methods:
- Two-dimensional polyacrylamide gel electrophoresis for enzyme separation.
- Histochemical staining on cellulose acetate membranes for substrate specificity analysis.
- Classification based on substrate hydrolysis (BAEE, BAPNA, poly-L-lysine), inhibitor sensitivity, and androgen dependence.
Main Results:
- Twenty-one proteolytically active esteroproteases were identified.
- Enzymes were categorized into four main groups based on substrate specificity.
- These groups were further resolved into eight subgroups based on inhibitor sensitivity and androgen dependence.
Conclusions:
- The male mouse submaxillary gland harbors at least eight distinct esteroprotease groups.
- These esteroproteases exhibit varied substrate specificities and regulatory properties.
- This detailed classification provides a foundation for further functional studies.