Related Experiment Video
Updated: Jul 23, 2026

The Lambda Select cII Mutation Detection System
Published on: April 26, 2018
Testing of sunset yellow and orange II for genotoxicity in different laboratory animal species
J Wever1, R Münzner, H W Renner
1Federal Research Centre for Nutrition, Karlsruhe, Federal Republic of Germany.
Abstract:
The azo dyes Sunset Yellow and Orange II were gavaged to rodent species to check bile, urine, and fecal extracts for possible mutagenic activity in the Ames test or in bone marrow cells for clastogenicity using cytogenetic test systems. After oral application the dyes showed a negative response in bile, excrements, and bone marrow. When an exogenous metabolic activation was performed, increased revertant numbers using Salmonella strain TA100 were obtained only in fecal extracts of Sunset Yellow-treated animals. It is concluded that no genotoxic harm is to be expected from the ingestion of Sunset-Yellow or Orange II.
Related Concept Videos
In-vitro Mutagenesis
In vitro Mutagenesis
Mutagenicity and Carcinogenicity
Toxicity Testing in Animals

