Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Key Techniques in Microbiology01:19

Key Techniques in Microbiology

3.0K
Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
3.0K
Methods for Controlling Microbial Growth01:29

Methods for Controlling Microbial Growth

2.6K
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
2.6K
Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

1.6K
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
1.6K
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

33
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
33
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

2.0K
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
2.0K
Need for Obtaining Pure Cultures01:29

Need for Obtaining Pure Cultures

2.6K
Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular characterisation of Toxoplasma gondii Mre11 reveals unique structural features and potential as a therapeutic target.

Scientific reports·2025
Same author

GCN5a is a telomeric lysine acetyltransferase whose loss primes <i>Toxoplasma gondii</i> for latency.

mSphere·2025
Same author

Toxoplasma gondii RAD51 recombinase is required to overcome DNA replication stress and its inactivation leads to bradyzoite differentiation.

DNA repair·2025
Same author

<i>Toxoplasma gondii</i> RAD51 recombinase is required to overcome DNA replication stress and its inactivation leads to bradyzoite differentiation.

bioRxiv : the preprint server for biology·2025
Same author

Cytosolic companionship: Rickettsia connects with the endoplasmic reticulum.

The Journal of cell biology·2025
Same author

Mechanisms of lipid homeostasis in the Coxiella Containing Vacuole.

Biochemical Society transactions·2025

Related Experiment Video

Updated: Mar 31, 2026

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
13:39

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Published on: July 7, 2023

19.5K

Preventing friendly fire in the war on microbes.

William J Sullivan1, Stacey D Gilk2

  • 1Department of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, IN 46202, USA. Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA. wjsulliv@iu.edu.

Science Translational Medicine
|October 30, 2015
PubMed
Summary

Targeting a specific chromatin-remodeling protein could potentially treat lung injury caused by Pseudomonas aeruginosa infection. This approach offers a new strategy for managing bacterial lung infections.

More Related Videos

Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
11:43

Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors

Published on: May 31, 2012

168.7K
Antimicrobial Characterization of Advanced Materials for Bioengineering Applications
08:08

Antimicrobial Characterization of Advanced Materials for Bioengineering Applications

Published on: August 4, 2018

22.9K

Related Experiment Videos

Last Updated: Mar 31, 2026

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
13:39

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Published on: July 7, 2023

19.5K
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
11:43

Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors

Published on: May 31, 2012

168.7K
Antimicrobial Characterization of Advanced Materials for Bioengineering Applications
08:08

Antimicrobial Characterization of Advanced Materials for Bioengineering Applications

Published on: August 4, 2018

22.9K

Area of Science:

  • Pulmonary Medicine
  • Microbiology
  • Molecular Biology

Background:

  • Pseudomonas aeruginosa is a significant opportunistic pathogen causing severe lung infections.
  • Lung injury associated with P. aeruginosa infection presents a major clinical challenge.
  • Understanding host-pathogen interactions at a molecular level is crucial for developing novel therapies.

Purpose of the Study:

  • To investigate the role of chromatin-remodeling proteins in the host response to P. aeruginosa lung infection.
  • To explore the therapeutic potential of targeting specific chromatin-remodeling proteins for mitigating P. aeruginosa-induced lung injury.

Main Methods:

  • Utilized a murine model of P. aeruginosa pneumonia.
  • Assessed lung injury markers and inflammatory responses.
  • Investigated the expression and function of a key chromatin-remodeling protein in lung epithelial cells.

Main Results:

  • The study identified a specific chromatin-remodeling protein implicated in the host's response to P. aeruginosa.
  • Modulating this protein's activity significantly reduced lung inflammation and injury.
  • Findings suggest a direct link between chromatin remodeling and bacterial pathogenesis in the lung.

Conclusions:

  • Targeting the identified chromatin-remodeling protein represents a promising therapeutic strategy.
  • This approach may offer a novel way to subvert P. aeruginosa-induced lung injury.
  • Further research into chromatin remodeling in infectious lung diseases is warranted.