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The complete islet amyloid polypeptide precursor is encoded by two exons.

S Mosselman1, J W Höppener, C J Lips

  • 1Institute of Molecular Biology and Medical Biotechnology, Utrecht, The Netherlands.

FEBS Letters
|April 10, 1989
PubMed
Summary

Researchers identified the genetic sequences for islet amyloid polypeptide (IAPP) precursors in human insulinomas. This finding advances understanding of IAPP

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Area of Science:

  • Molecular Biology
  • Genetics
  • Endocrinology

Background:

  • Islet amyloid polypeptide (IAPP) is a peptide subunit of amyloid deposits observed in type 2 diabetic patients and insulinomas.
  • The human IAPP gene was previously isolated.

Purpose of the Study:

  • To determine the nucleotide sequences of the human IAPP precursor and its corresponding gene segments.
  • To elucidate the genetic structure encoding IAPP.

Main Methods:

  • Isolation and sequencing of human insulinoma cDNA encoding the complete IAPP precursor.
  • Analysis of the corresponding human IAPP gene structure.

Main Results:

  • The nucleotide sequences for an 89-amino acid pre-pro-IAPP precursor were determined.

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  • The human IAPP gene consists of at least three exons, with two exons encoding pre-pro-IAPP located approximately 5 kb apart.
  • A putative signal sequence at the N-terminus of the precursor indicates IAPP is a secreted protein.

    • Conclusions:

    • The genetic information for the complete IAPP precursor has been elucidated.
    • The gene structure suggests a complex transcriptional or processing mechanism for IAPP.
    • Understanding IAPP gene organization provides insights into amyloid formation in diabetes and tumors.