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Related Experiment Videos

Semen manipulation: improved sperm recovery and function with a two-layer Percoll gradient.

R D McClure1, L Nunes, R Tom

  • 1Department of Urology, University of California, San Francisco.

Fertility and Sterility
|May 1, 1989
PubMed
Summary

The Percoll density gradient technique offers superior semen preparation compared to the swim-up method. It enhances sperm yield, motility, and function, improving outcomes for fertility treatments.

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Area of Science:

  • Reproductive biology
  • Andrology
  • Sperm preparation techniques

Background:

  • Semen preparation is crucial for assisted reproductive technologies.
  • Traditional methods like swim-up have limitations in sperm recovery and function.
  • Optimizing sperm selection is key to improving fertilization rates.

Purpose of the Study:

  • To compare the efficacy of a two-layer Percoll density gradient technique with the swim-up technique for semen preparation.
  • To evaluate the impact of each technique on sperm yield, motility, and function.
  • To determine if Percoll gradient improves sperm quality in various patient groups.

Main Methods:

  • A comparative study involving 128 men with different semen parameters.
  • Semen samples were processed using both the Percoll density gradient and swim-up techniques.

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  • Sperm yield, motility, and function (sperm penetration assay, fertility index) were assessed.

    • Main Results:

    • The Percoll technique significantly improved sperm yield, motility, and motile sperm count in normospermic, oligospermic, and asthenospermic samples.
    • In oligoasthenospermic samples, Percoll only improved percent motility.
    • Percoll processing enhanced sperm function, with 51% of samples negative on the sperm penetration assay becoming positive.

    Conclusions:

    • The Percoll density gradient is an effective method for semen preparation, yielding greater recovery of motile sperm.
    • This technique improves sperm function, as evidenced by improved sperm penetration assay and fertility index results.
    • Percoll density gradient offers an advantage over the swim-up technique for semen manipulation in fertility contexts.