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Updated: Mar 31, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Complete mitochondrial genome of the Pseudopleuronectes yokohamae (Pleuronectiforll: Pleuronectidae)
Fengrong Zheng1, Hongzhan Liu2, Yongqiang Zhang1
1a Ecology laboratory of the First Institute of Oceanography SOA , Qingdao , P.R. China.
Abstract:
In this study, the complete mitochondrial genome of the Pseudopleuronectes yokohamae was sequenced. The mitochondrial genome is 17 383 bp in length, consisting of 13 protein-coding genes, 2 ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs) and a control region (D-loop). The gene order and composition of P. yokohamae mitochondrial genome was similar to that of most other vertebrates. Two rRNA genes for 12S rRNA (949 bases) and 16S rRNA (1716 bases) are located between tRNA-Phe (GAA) and tRNA-Leu (TAA) and separated by the tRNA-Val. The control region of P. yokohamae, is located after tRNA-Pro and found to be 1679 bp. The overall nucleotides base composition of the heavy strand is A (27.55%), T (26.45%), G (28.75%), C (17.26%). With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. The 22 tRNA genes could be folded into a typical clover-leaf secondary structure. The phylogenetic trees consistently show that P. yokohama, Kareius bicoloratus and Platichthys stellatus from the same genus clustered into one branch. The complete mitogenome sequence information of P. yokohamae would provide useful data for further studies on population genetics and molecular systematics.
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