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Updated: Mar 31, 2026
Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
Bone regeneration by low-level laser therapy and low-intensity pulsed ultrasound therapy in the rabbit calvarium
Ahmet Hüseyin Acar1, Ümit Yolcu2, Sedat Altındiş3
1Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bezmialem Vakif University, Istanbul, Turkey.
Objective:
We evaluated the efficacy of low-level laser therapy (LLLT) and low-intensity pulsed ultrasound (LIPUS), alone and in combination, in triggering new bone formation.
Study Design:
Sixteen New Zealand white rabbits were given two calvarial defects by using a 6-mm trephine bur, then divided into four treatment groups: control, LLLT, LIPUS, and LLLT+LIPUS. The LLLT and LIPUS groups were treated three times a week for two weeks. The LLLT+LIPUS group received each treatment on the same day, 12h apart, three days a week for two weeks. The animals were sacrificed after three weeks.
Results:
LLLT and LIPUS, alone and in combination, enhanced new bone formation in comparison to the untreated controls after three weeks (P<0.05); the combined therapy did not produce an additive effect.
Conclusions:
Our results demonstrate the efficacy of LLLT or LIPUS in triggering bone regeneration. Therapeutic dose and duration requires further study.
