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Chromosomal transformation of Escherichia coli recD strains with linearized plasmids
C B Russell1, D S Thaler, F W Dahlquist
1Institute of Molecular Biology, University of Oregon, Eugene 97403.
Journal of Bacteriology
|May 1, 1989
Abstract:
Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transferred to the chromosome by homologous recombination, whereas plasmid markers not in the region of homology were lost.