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Production scale purification of biosynthetic human insulin by reversed-phase high-performance liquid chromatography
E P Kroeff1, R A Owens, E L Campbell
1Department of Biosynthetic Isolation and Purification Development, Lilly Research Laboratories, Indianapolis, IN 46285.
Journal of Chromatography
|January 6, 1989
Abstract:
A process based on reversed-phase high-performance liquid chromatography (RP-HPLC) has been developed for the purification of biosynthetic human insulin (BHI). The RP-HPLC procedure has been successfully integrated into the multimodal chromatographic production process used to purify kilogram quantities of BHI. Axial compression column technology was used in the scale-up process. The RP-HPLC procedure yields an insulin product having high chemical purity and full biological activity.