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Optimizing Multiple Analyte Injections in Surface Plasmon Resonance Biosensors with Analytes having Different
Massinissa Si Mehand1, Bala Srinivasan1, Gregory De Crescenzo1
1Department of Chemical Engineering, École Polytechnique de Montréal. P.O. Box 6079, Centre-ville Station, H3C 3A7 Montréal, Québec, Canada.
Scientific Reports
|October 31, 2015
Summary
This study introduces a new method for surface plasmon resonance (SPR) biosensing that bypasses the need for refractive index increment measurements. This approach enhances throughput for analyzing small molecule interactions without additional experiments.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Biophysics
Background:
- Surface plasmon resonance (SPR) biosensors are vital for studying molecular interactions.
- Current SPR methods require refractive index increment data for accurate analysis of small molecules, necessitating extra experiments.
- Increasing throughput in SPR experiments is a key challenge.
Purpose of the Study:
- To develop a method for SPR analysis of small molecules that eliminates the need for separate refractive index increment measurements.
- To improve the efficiency and throughput of SPR-based screening of small molecular weight compounds.
- To enable accurate kinetic parameter determination without prior knowledge of individual compound properties.
Main Methods:
- Simultaneous injection of multiple small molecular weight compounds over a single SPR sensor surface.
- Introduction of a global parameter representing the ratio of saturating signals for each molecule.
- Mathematical modeling to extract kinetic parameters from the SPR response.
Main Results:
- Successfully identified kinetic parameters with comparable confidence intervals to traditional methods.
- Eliminated the requirement for an additional experiment to determine refractive index increments.
- Demonstrated the feasibility of analyzing multiple small molecules simultaneously with enhanced throughput.
Conclusions:
- The proposed method simplifies SPR-based kinetic analysis of small molecules.
- This advancement significantly increases the throughput of SPR screening.
- The new approach offers a more efficient way to study molecular interactions using SPR biosensors.
