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A simple hemagglutination method for the detection of cell surface antigens
D Kokkinopoulos1, S Perez, M Papamichail
1Department of Immunology, Hellenic Anticancer Institute, Athens, Greece.
Journal of Immunological Methods
|April 21, 1989
Summary
A novel hemagglutination assay offers a rapid and reproducible method for detecting cell surface antigens. This technique aids in identifying cell subpopulations and screening hybridomas, correlating well with immunofluorescence methods.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Accurate detection of cell surface antigens is crucial for understanding cellular functions and disease states.
- Existing methods for antigen detection can be time-consuming or require specialized equipment.
Purpose of the Study:
- To develop a rapid, easy, and reproducible hemagglutination method for detecting cell surface antigens.
- To enable the determination of cell subpopulations and screening of monoclonal antibody-producing hybridomas.
Main Methods:
- A hemagglutination assay using a 96-well microtiter plate.
- Cells labeled with monoclonal antibodies are reacted with antibody-coated human erythrocytes.
- Serial dilutions are used to estimate the percentage of antigen-positive cells.
Main Results:
- The hemagglutination method demonstrated rapid and reproducible detection of cell surface antigens.
- The assay effectively facilitated the identification of cell subpopulations.
- Results showed good correlation with conventional immunofluorescence techniques.
Conclusions:
- The described hemagglutination method is a valuable tool for cell surface antigen detection.
- This assay provides an efficient alternative for hybridoma screening and cell subpopulation analysis.
- The method's ease of use and reproducibility make it suitable for various immunological applications.