Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

4.0K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
4.0K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

7.6K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preclinical evaluation of cysteine protease-inhibitor aloxistatin (E64d) for heart failure therapy.

Journal of molecular medicine (Berlin, Germany)·2026
Same author

Time- and spatially resolved LNA delivery via thermally controlled SPION technology.

Molecular therapy. Nucleic acids·2026
Same author

The conjugation-resistant bile acid norUDCA cures liver fibrosis but impairs systemic energy metabolism.

Molecular metabolism·2026
Same author

Higher sensitivity to ouabain-induced toxicity in human induced pluripotent stem cell-derived cardiomyocytes than human adult heart tissue despite similar Na<sup>+</sup>/K<sup>+</sup>-ATPase pump current amplitudes.

British journal of pharmacology·2026
Same author

Macrophage-specific circular RNA circHIPK2, inflammation, and fibrosis after myocardial infarction.

European heart journal·2026
Same author

Increase in mechanical load and pro-fibrotic stimulation leads to fibrotic and hypertrophic remodeling in porcine living myocardial slices.

Scientific reports·2025

Related Experiment Video

Updated: Mar 31, 2026

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
07:09

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Published on: April 30, 2017

13.1K

Development of Long Noncoding RNA-Based Strategies to Modulate Tissue Vascularization.

Jan Fiedler1, Kaja Breckwoldt2, Christian W Remmele3

  • 1Institute of Molecular and Translational Therapeutic Strategies, Hannover Medical School, Hannover, Germany; Integrated Research and Treatment Center Transplantation, Hannover Medical School, Hannover, Germany.

Journal of the American College of Cardiology
|October 31, 2015
PubMed
Summary

Researchers identified hypoxia-sensitive long noncoding RNAs (lncRNAs) crucial for endothelial cell function and blood vessel formation. These lncRNAs, LINC00323 and MIR503HG, are potential therapeutic targets for cardiovascular diseases.

Keywords:
angiogenesisendothelial cell biologyhypoxialncRNA

More Related Videos

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles
09:01

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Published on: September 27, 2013

11.6K
A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo
07:56

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Published on: August 28, 2014

13.0K

Related Experiment Videos

Last Updated: Mar 31, 2026

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
07:09

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Published on: April 30, 2017

13.1K
Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles
09:01

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Published on: September 27, 2013

11.6K
A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo
07:56

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Published on: August 28, 2014

13.0K

Area of Science:

  • Molecular Biology
  • Genomics
  • Cardiovascular Research

Background:

  • Long noncoding RNAs (lncRNAs) regulate gene expression but their role in human endothelial cells and angiogenesis is understudied.
  • Endothelial cells are critical for blood vessel formation and function.

Purpose of the Study:

  • To discover and functionally characterize hypoxia-sensitive lncRNAs in human endothelial cells.
  • To investigate the role of these lncRNAs in angiogenic processes.
  • To explore their potential as therapeutic targets for cardiovascular diseases.

Main Methods:

  • Next-generation RNA sequencing and microarray analysis to identify hypoxia-sensitive lncRNAs.
  • In vitro and ex vivo experiments to assess the angiogenic function of identified lncRNAs.
  • Gene silencing techniques to determine the impact of lncRNAs on endothelial cell behavior.

Main Results:

  • Identified and validated two hypoxia-sensitive lncRNAs, LINC00323 and MIR503HG, in endothelial cells.
  • Silencing these lncRNAs impaired angiogenesis, repressed growth factor signaling, and affected GATA2 expression.
  • Loss of these lncRNAs disrupted endothelial cell-cycle control and inhibited capillary formation.
  • Demonstrated the clinical relevance of these lncRNAs in a human engineered heart tissue model.

Conclusions:

  • An expression atlas of human hypoxia-sensitive lncRNAs was established.
  • Two specific lncRNAs (LINC00323 and MIR503HG) were found to be essential for endothelial cell biology.
  • These lncRNAs represent promising therapeutic targets for cardiovascular diseases.