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Three-dimensional imaging in fluorescence by confocal scanning microscopy
G J Brakenhoff1, H T van der Voort, E A van Spronsen
1Department of Electrical Microscopy and Molecular Cytology, University of Amsterdam, The Netherlands.
Journal of Microscopy
|February 1, 1989
Abstract:
The improved resolution and sectioning capability of a confocal microscope make it an ideal instrument for extracting three-dimensional information especially from extended biological specimens. The imaging properties, also with finite detection pinholes are considered and a number of biological applications demonstrated.