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Updated: Mar 31, 2026
06:58
Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
Published on: October 7, 2021
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Dynamics of co-translational protein targeting.
Margaret M Elvekrog1, Peter Walter1
1The Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, United States.
Current Opinion in Chemical Biology
|October 31, 2015
Summary
The signal recognition particle (SRP) machinery guides proteins to membranes. This study reveals how SRP
Area of Science:
- Molecular biology
- Cell biology
- Biochemistry
Background:
- Most membrane and secretory proteins are co-translationally delivered to translocation channels via the signal recognition particle (SRP) and its receptor.
- This essential co-translational machinery is conserved across all life forms, with variations in its components and functions.
Purpose of the Study:
- To elucidate the mechanism of signal recognition particle (SRP) function.
- To investigate the conformational dynamics of Escherichia coli SRP during the protein targeting process.
Main Methods:
- Focus on findings related to Escherichia coli SRP's conformational dynamics.
- Analysis of the targeting cycle and regulatory checkpoints.
Main Results:
- Detailed insights into the conformational changes of SRP during ribosome binding and targeting.
- Identification of key regulatory steps in SRP-mediated protein translocation.
Conclusions:
- Understanding SRP conformational dynamics is crucial for comprehending protein targeting.
- SRP plays a critical role in regulating ribosome engagement with the membrane translocation machinery.
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