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Related Experiment Videos

R-body-producing bacteria.

F R Pond, I Gibson, J Lalucat

    Microbiological Reviews
    |March 1, 1989
    PubMed
    Summary
    This summary is machine-generated.

    R bodies, once thought to be limited to paramecia endosymbionts, are now found in diverse bacteria. Their structure and genetic basis are being elucidated through cloning and expression studies in E. coli.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • R bodies were historically identified as diagnostic features of kappa endosymbionts in *Paramecium* species.
    • Their presence was previously believed to be restricted to the cytoplasm of specific *Paramecium aurelia* complex species.

    Purpose of the Study:

    • To investigate the expanded distribution of R bodies beyond *Paramecium* endosymbionts.
    • To explore the structural and genetic underpinnings of R body synthesis and assembly.

    Main Methods:

    • Characterization of R bodies from various *Paramecium* species and free-living bacteria.
    • Cloning and expression of genetic determinants for type 51 R bodies in *Escherichia coli*.
    • Analysis of R body polypeptide composition and genetic encoding (plasmid vs. bacteriophage).

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    Main Results:

    • R bodies are now identified in diverse organisms, including free-living bacteria (*Pseudomonas*, *Rhodospirillum*) and other *Paramecium* species.
    • Type 51 R body synthesis in *E. coli* requires three polypeptides, with one undergoing post-translational modification to yield 12 major structural subunits.
    • R bodies are encoded by extrachromosomal elements in kappa species, with plasmids or bacteriophages implicated, though not universally present in all R-body-producing bacteria.

    Conclusions:

    • The known diversity of R body-producing organisms has significantly expanded.
    • The genetic basis of R body synthesis, particularly for type 51, can be studied heterologously in *E. coli*.
    • The evolutionary relationships and precise genetic mechanisms across different R body types require further investigation.