Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phenotypic and genotypic analysis of <i>Candida albicans</i> vaginal isolates reveals that <i>ECE1</i> expression underpins pathogenicity.

Infection and immunity·2026
Same author

Immunization with the pan-fungal vaccine, NXT-2a, reduces fungal burden following serial intravaginal challenges with C. albicans in a non-human primate model of experimental vulvovaginal candidiasis.

PloS one·2026
Same author

Phage-derived endolysin prevents dissemination of methicillin resistant <i>Staphylococcus aureus</i> during oral co-infection with <i>Candida albicans</i>.

Antimicrobial agents and chemotherapy·2026
Same author

A mechanistic framework linking the oral microbiome to Alzheimer's disease through neuroinflammation.

Journal of Alzheimer's disease : JAD·2026
Same author

Secreted factors of <i>Staphylococcus aureus</i> promote co-invasion with <i>Candida albicans</i> by inducing hypha formation and invasion.

Applied and environmental microbiology·2026
Same author

Candida albicans facilitates Porphyromonas gingivalis phagocytosis and combined exposure stimulates predominantly M1 response in macrophages in vitro.

Biochemical and biophysical research communications·2025

Related Experiment Video

Updated: Mar 31, 2026

Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device
11:14

Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device

Published on: December 14, 2017

11.3K

In Vitro Models for Candida Biofilm Development.

Bastiaan P Krom1, Hubertine M E Willems2

  • 1Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Free University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands. b.krom@acta.nl.

Methods in Molecular Biology (Clifton, N.J.)
|November 1, 2015
PubMed
Summary

This study explores various in vitro models for studying Candida biofilms on medical devices. It details methods for optimizing biofilm formation and analysis, aiding in the development of new treatments.

Keywords:
Amsterdam Active Attachment modelBiofilmBiofluxMicrotiter plate assaySusceptibility test ing

More Related Videos

Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging
08:31

Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging

Published on: January 27, 2015

13.7K
A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
06:50

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

Published on: September 16, 2022

3.7K

Related Experiment Videos

Last Updated: Mar 31, 2026

Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device
11:14

Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device

Published on: December 14, 2017

11.3K
Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging
08:31

Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging

Published on: January 27, 2015

13.7K
A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
06:50

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

Published on: September 16, 2022

3.7K

Area of Science:

  • Microbiology
  • Biomedical Engineering
  • Medical Mycology

Background:

  • Candida spp. biofilms on medical devices pose a growing clinical challenge.
  • In vitro models are crucial for studying biofilm formation and testing anti-biofilm compounds.
  • Existing models vary in complexity and throughput for biofilm analysis.

Purpose of the Study:

  • To describe optimized in vitro models for Candida biofilm development and analysis.
  • To evaluate different model systems for their suitability in susceptibility studies and mechanistic investigations.
  • To present advanced models for simulating realistic biofilm conditions and dynamic processes.

Main Methods:

  • Optimized microtiter plate (96-well) model for biofilm formation and susceptibility testing.
  • Amsterdam Active Attachment (AAA) model (24-well) for simulating adhesion, proliferation, and maturation.
  • Bioflux microfluidics system for high time-resolution dynamic process studies.

Main Results:

  • Optimized microtiter plate conditions facilitate C. albicans biofilm formation for microscopy and molecular studies.
  • The AAA model effectively simulates key biofilm stages on various surfaces, enabling interaction studies.
  • The Bioflux system allows real-time observation of dynamic biofilm formation and molecular mechanisms.

Conclusions:

  • Diverse in vitro models, from simple to complex, are available for Candida biofilm research.
  • These models support the development of novel anti-Candida biofilm strategies and deepen understanding of biofilm biology.
  • Advanced models like AAA and Bioflux offer enhanced realism for studying complex biofilm dynamics and interactions.