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The Fungal Biome of the Oral Cavity

Jyotsna Chandra1, Mauricio Retuerto1, Pranab K Mukherjee1

  • 1Center for Medical Mycology and Department of Dermatology, University Hospitals Case Medical Center and Case Western Reserve University, 11100 Euclid Ave, Cleveland, OH, 44106, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 1, 2015
PubMed

Insights

This study details methods for characterizing the oral mycobiome, the fungal community in the mouth. Understanding these fungi is crucial for oral health, especially in HIV patients.

Area of Science:

  • Microbiology
  • Immunology
  • Mycology

Background:

  • Oral microbiota impacts health and diseases like gingivitis, periodontitis, and oral candidiasis.
  • Oral microbes are linked to systemic conditions such as cancer and respiratory infections.
  • Limited knowledge exists on oral microbial interactions and host immune system influence.

Purpose of the Study:

  • To describe methods for characterizing the oral mycobiome.
  • To focus on the fungal component of oral microbiota, particularly in the context of HIV.
  • To advance understanding of the oral fungal community in health and disease.

Main Methods:

  • Characterization of the oral mycobiome.
  • Focus on fungal community analysis.
  • Methods applied in health and disease, with emphasis on HIV-associated oral candidiasis.

Main Results:

  • Recent characterization of the oral mycobiome in health and disease.
  • Specific focus on HIV-related oral conditions.
  • Detailed description of methodologies employed by the research group.

Conclusions:

  • Characterizing the oral mycobiome is essential for understanding oral health and disease.
  • The study provides a methodological framework for oral fungal community analysis.
  • Further research into the oral mycobiome, especially in immunocompromised individuals, is warranted.
Keywords:
AmplificationFungal DNA extractionFungusMycobiomeOral microbiotaSequencing

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