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Detection of a Myc-associated protein by chemical cross-linking
1Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Molecular and Cellular Biology
|February 1, 1989
Abstract:
A single nuclear protein (Myc-associated protein) can be specifically cross-linked to avian Myc proteins by treatment of nuclei or cells with the reversible cross-linker dimethyl 3,3'-dithiobis-propionimidate. Myc-associated protein has a molecular weight of approximately 500,000, is not detectably phosphorylated and, in contrast to Myc, has a long apparent half-life of greater than 3 h.