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Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
Published on: July 18, 2013
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Time-Lapse Fluorescence Microscopy of Budding Yeast Cells
Arun Kumar1,2, Manuel Mendoza3,4
1Centre for Genomic Regulation (CRG), Dr. Aiguader 88, Barcelona, 08003, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|November 1, 2015
Summary
Researchers developed a method to visualize multiple cell division events in yeast using time-lapse fluorescence microscopy. This technique allows simultaneous tracking of key processes like ring contraction and membrane ingression in living cells.
Area of Science:
- Cell Biology
- Microscopy
- Molecular Biology
Background:
- Green fluorescent protein (GFP) enables visualization of cellular processes in living cells.
- Development of multicolor fluorophores allows simultaneous tracking of distinct subcellular events.
- Cytokinesis in Saccharomyces cerevisiae involves an actomyosin ring driving plasma membrane ingression.
Purpose of the Study:
- To describe a method for visualizing multiple events during cytokinesis in budding yeast.
- To enable simultaneous observation of distinct cytokinesis steps in living cells.
- To facilitate a comprehensive understanding of eukaryotic cell division mechanisms.
Main Methods:
- Utilized time-lapse fluorescence microscopy.
- Employed green fluorescent protein (GFP) and other fluorophores for multi-event visualization.
- Focused on the budding yeast Saccharomyces cerevisiae as a model organism.
Main Results:
- Successfully visualized multiple distinct events during cytokinesis.
- Enabled simultaneous tracking of processes such as actomyosin ring contraction and membrane ingression.
- Demonstrated the utility of the method for studying cell division in real-time.
Conclusions:
- The developed method allows for simultaneous visualization of key cytokinesis events in living yeast cells.
- This technique is crucial for a complete understanding of the molecular mechanisms underlying eukaryotic cell division.
- Advances in multicolor fluorescence microscopy are key to dissecting complex cellular processes.
